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Why did you discard any enzymes that cut the plasmid in the shaded plasmid replication sequence?
Discard any enzymes that cut the plasmid in the shaded plasmid replication sequence. You don’t want to cut out this particular gene, because it is necessary for the bacteria to replicate itself. 6. Next, compare the enzymes you chose in step 5 against the cell DNA strip.
Why was it important to find an enzyme that would cut the plasmid at only one site what could happen if the plasmid was to cut at more than one site?
What could happen if the plasmid were cut at more than one site? You simply want to open the circular DNA so that the human DNA can be inserted into the circle. If the enzymes cut at multiple spots, then you would get multiple fragments.
Why is it important that restriction enzymes leave sticky ends when they cut DNA to produce a recombinant plasmid?
For this reason, enzymes that leave single-stranded overhangs are said to produce sticky ends. Sticky ends are helpful in cloning because they hold two pieces of DNA together so they can be linked by DNA ligase.
What happens to the plasmid when you cut it?
What happens to the plasmid when you cut it? When the plasmid is cut, since it is circular and there is only one restriction site, it will result in one linear piece of DNA. When Chromosome 17 is cut, it will result in the uninterrupted gene and two end pieces, resulting in 3 pieces of DNA.
Why might it be important to cut the DNA strand as?
Why might it be important to cut the DNA strand as closely to the desired gene as possible? Cutting the DNA close to the desired gene is necessan, so that undesired sequences are left out and the sticky ends find each other.
Why was it important to discard any enzymes?
Why was it important to discard any enzymes that cut the plasmid at the replication site? (If the plasmid were cut at the replication site, it would not be able to reproduce and transfer genetic information to its host bacterial cell.)
Why is the sticky ends important?
Sticky ends are more useful in molecular cloning because they ensure that the human DNA fragment is inserted into the plasmid in the right direction. The ligation process, or fusing of DNA fragments, requires less DNA when the DNA have sticky ends.
Why do some restriction enzymes cut with blunt end and others with a sticky ends?
After digestion of a DNA with certain restriction enzymes, the ends left have one strand overhanging the other to form a short (typically 4 nt) single-stranded segment. This overhang will easily re-attach to other ends like it, and are thus known as “sticky ends”.
Why do scientists use the same enzyme to remove the insulin and cut the plasmid open?
A bacterial plasmid is cut open using the same restriction enzyme . Restriction enzymes leave ‘sticky ends’, where one of the two DNA strands is longer than the other. Using the same restriction enzyme to cut both the human DNA and bacterial plasmid results in complementary sticky ends that join by base pairing.
Do you think restriction enzymes would be used to cut DNA from other organisms?
Restriction enzymes dismantle foreign DNA by cutting it into fragments. This disassembling process is called restriction. Recombinant DNA technology relies on restriction enzymes to produce new combinations of genes.